畜产品安全与毒害物质残留研究进展

论述了畜产品安全及其目前畜产品中的兽药、激素、重金属、生物毒素、农药、微生物等毒害物质的残留，并对畜产品安全现状及存在的问题进行了分析。     

杏胚珠培养及幼胚子叶不定芽诱导研究

以盛花后25、35、50、55、60d的凯特杏胚珠为外植体,研究胚珠培养的适宜条件;选择发育一致的幼胚子叶,研究不定芽的发生条件。结果表明,SH培养基是凯特杏胚珠培养的适宜培养基;盛花后25d的凯特杏胚珠(PF=0),仅采用生长培养不足以发育成可见胚,试验采用诱导方式获得了可见胚,诱导出胚率为80%;以MS为基本培养基,BA与NAA结合诱导能力强;但这种胚小且瘦弱,继续生长培养,利用幼胚子叶诱导不定芽。在幼胚子叶诱导不定芽方面,盛花后25、35d取样的凯特杏胚珠,培养后获得幼胚子叶,诱导不定芽的适宜TDZ质量浓度为1.25mg/L,诱导出芽率分别为85.00%、80.00%;盛花后50、55d取样的材料,适宜TDZ质量浓度为2.50mg/L,出芽率分别为65.00%、60.00%,而盛花后60d取样的材料,适宜TDZ质量浓度为5.0mg/L,出芽率为61.11%,不定芽主要发生在子叶的近轴面、胚轴连接处及子叶近轴端两侧;在相同培养基中,25℃进行21d暗培养,盛花后35、50、55、60d材料出芽率分别为72.22%、65.00%、60.00%、55.56%;4℃下28d暗培养,35、50、55、60d依次为59.09%,71.42%、77.78%、86.36%;对不定芽进行伸长、生根与快繁培养,获得了健康正常的植株。通过以上研究,建立了凯特杏幼胚4级培养体系,即(1)胚珠培养,获得可见胚(1级培养);(2)幼胚生长培养(胚培养),获得了健壮幼胚(2级培养);(3)幼胚子叶再生,获得不定芽(3级培养);(4)不定芽组培苗快繁(4级培养)。     

分子修饰前后人参皂苷体外抗马立克氏病毒鸡胚成纤维细胞模型的建立

用雏鸡将马立克氏病毒(MDV)血毒复壮,分离发病鸡淋巴细胞并接种于鸡胚成纤维细胞,观察其病变。获得适应鸡胚成纤维细胞(CEF)的MD强毒,通过电镜观察、琼脂扩散实验进一步鉴定病毒,并建立了MDV感染CEF细胞模型,为研究人参皂苷及其衍生物的体外抗病毒作用及其机制奠定基础。     

Cloning,Expression and Bioinformatics Analysis of Enterococcus faecalis from Northeast Black Bear

In order to detect Enterococcus faecalis endocarditis antigen (EfaA) of bear that was used to disclose the infected Northeast Black bear immediately, a pair of PCR primers was synthesized by EfaA gene of Enterococcus faecalis that was recorded in GenBank (accession number:U03756.1) and the target fragment was 689 bp. The PCR product was cloned by pMD19-T vector, then was transferred to Escherichia coli DH5α competent cells and the positive clones were filtered. Recombinant plasmid (pMD19-T-EfaA) was extracted. Identification of PCR and digestion, sequencing, the structure prediction of proteins were operated. The results showed that EfaA gene was cloned successfully, and the gene homology with ATCC 29212 was 100.0%. pMD19-T-EfaA was constructed successfully, structure of proteins was predicted. This study provided theoretical basis for diagnostic methods of Enterococcus faecalis and laid basis for prevention and control of Northeast Black bear disease.     

Quantitative dynamics on stimulating regeneration and sowing seedlings of Larix gmelinii in Daxing''''an Mountains

Introduction4LarixgmeliniiisoneofmajortimberspeciesinthenortheastDaxing'anMountainsinChina.Itplaysanimportantroleinprovidinglumber,maintainingthenaturalenvironmentinthisregionandadjustingecologicalequilibrium.Alargenumberofburnedareasandcut-overareasneedtoregenerateeveryyear.Larixgmeliniihasgreatnaturalregenerationcapabilityandenoughseedsources,andalmostmeetsabumperharvestyeareverythreeyears(Xuetal.1992;Shan1990;Xuetal.1993).Someburnedandloggedareaswouldberapidlyrestoredthroughafewofassista…     

硫酸化人参总皂苷的制备及其对鸡外周血淋巴细胞增殖的影响

用氯磺酸-吡啶试剂制备了硫酸化人参总皂苷衍生物，采用红外光谱仪检测到制备的硫酸化人参总皂苷衍生物中在1230cm^-1和810cm^-1有硫酸酯键的特征吸收峰。为了初步探讨硫酸化人参总皂苷的免疫学活性．以MTT法测定了人参总皂苷及其衍生物对鸡外周血淋巴细胞增殖的影响。结果表明，150μg／m1人参总皂苷和衍生物均能显著抑制鸡外周血淋巴细胞的增殖（P〈0．05），10μg／ml的衍生物能极显著促进淋巴细胞增殖，与同浓度人参总皂苷相比活性极显著增强（P〈0．01）。实验结果提示，对人参总皂苷进行硫酸化修饰是可行的，经硫酸化后人参总皂苷衍生物的免疫活性明显增强。     

利用根尖微核技术对白河水质的研究

利用蚕豆根尖微核检测技术对南阳市区白河河段不同样点的河水进行检测.南阳大桥处的流水污染程度较轻,滨河路尽头距橡胶坝约200 m处的河边流水污染程度重,其它各处均为中度污染.严格监管南阳市区白河两岸的工厂、企业、养殖户等的排污及治理情况,加强对游人、垂钓者等的道德、法制宣传教育,采取相应的无损市民生命安全、可以维持生态系统平衡的河水治污方式净化河水.     

彩色马蹄莲高效组培增殖条件研究

以彩色马蹄莲的品种‘Golden Affairs'为试材,在9种培养基上进行增殖培养,分20d、30d、40d三阶段称重,计算增重率,研究激素对增殖的影响及适宜的增殖周期。结果表明:高浓度BA不利于丛生芽增殖,可促进苗的形成;丛生芽的增殖则受BA/NAA的比值调节;30d为增殖周期进行培养,可获得最大增殖率。     

水稻施用硅钙肥试验总结

为了研究硅钙肥对水稻生育及产量的影响,采用寿益牌硅钙肥进行大区对比试验,供试的水稻品种为辽粳9号。试验结果表明:硅钙肥可促进水稻分蘖,茎秆高大粗壮,增强抗逆性,增产5.7%～7.3%,增收31.20、18.60元/667 m2。